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Year : 2015  |  Volume : 8  |  Issue : 2  |  Page : 115-122

Genotyping of Blastocystis hominis symptomatic isolates and kinetics of associated local CD3 and CD20 cell infiltrate

1 Department of Medical Parasitology, Mansoura University, Mansoura, Egypt
2 Department of Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
3 Department of Microbiology, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Correspondence Address:
Manar S Azab
PhD, Department of Medical Parasitology, Faculty of Medicine, Mansoura University, 2 El-Gomhouria Street, Mansoura 35516
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/1687-7942.175009

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Background Pathogenicity of the protozoan parasite Blastocystis hominis is a subject of debate. It has been suggested that the pathogenic outcome may be linked to specific subtypes of Blastocystis spp. Studies on experimental infection in animals have reported varying degrees of illness depending on the used genotype. Objective This study was designed to identify a possible link between Blastocystis genotypes and gastrointestinal illness. In addition, the CD3 and CD20 local immune response to infection using symptomatic isolates was experimentally evaluated. Patients and methods Blastocystis- infected symptomatic (n = 51) and asymptomatic (n = 19) patients and irritable bowel syndrome patients (n = 32) were enrolled in the study, after exclusion of individuals who had other possible fecal pathogens. Restriction fragment length polymorphism was used for genotyping of isolates. Isolates from symptomatic cases were used for experimental infection, and immunohistochemical characterization of local CD3 and CD20 response was evaluated at two time intervals (groups A and B) after infection. Results Genotype 3 was the most common, being detected in 55.9% of all studied participants, and genotype 4 was the least common (9.8%). Symptomatic cases constituted 90% of genotype 1, 45.6% of genotype 3, 40% of genotype 4, and 20% of genotype 2. Genotype 2 was detected in 14.7% of all studied patients, with asymptomatic patients accounting for 60% of this isolate. Twenty-four isolates of genotype 3 occurred in 42.1% of irritable bowel syndrome patients. Rats euthanized after 7 (group A) and 14 days (group B) had higher CD3 and CD20 mean cell counts compared with control rats. The mean cell count of CD3 cells was statistically significantly higher in group A compared with group B, whereas CD20 cells in group B showed statistically significantly higher mean count compared with group A. Conclusion We suggest that both host and pathogen factors cooperate to express the pathogenic behavior of the parasite.

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