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Figure 2 Results of restriction fragment length polymorphism after digestion of a PCR-amplified product with endonuclease, AflIII and ApoI for Pfmdr1 gene (86Y). Lanes: L, molecular weight ladder; C, negative control; S1, S4, S6, S7, S8, S10, S11, S12, and S14, two fragments of 279 and 222 bp, indicating a mutant allele at codon 86 (86T); S2, S3, S5, S9, and S13, a single band of 500 bp indicating no mutation in the Pfmdr1 gene. |
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